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j gleeson  (Addgene inc)


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    Addgene inc j gleeson
    J Gleeson, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rfp+dcx/DCX-RFP+(Plasmid+%2332851)/pmc12309678-170-41-46
    Average 92 stars, based on 4 article reviews
    j gleeson - by Bioz Stars, 2026-09
    92/100 stars

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    Human ATAT1 colocalizes with doublecortin-bundled microtubules. A, HEK293 cells were transfected with an expression plasmid for GFP-α-tubulin, <t>RFP-DCX,</t> or GFP-ATAT1 for live green or red fluorescence microscopy. B, HEK293 cells were cotransfected with the expression plasmids for GFP-α-tubulin and RFP-DCX for live green and red fluorescence microscopy. Cross-talk between different channels was none or minimal (data not shown). C, same as B except that GFP-ATAT1 was expressed instead of GFP-α-tubulin. Similar results were obtained with mCherry-ATAT1 and GFP-DCX, although mCherry-ATAT1 was more enriched in the cytoplasm than GFP-ATAT1 (data not shown). Scale bar, 40 μm.
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    Human ATAT1 colocalizes with doublecortin-bundled microtubules. A, HEK293 cells were transfected with an expression plasmid for GFP-α-tubulin, RFP-DCX, or GFP-ATAT1 for live green or red fluorescence microscopy. B, HEK293 cells were cotransfected with the expression plasmids for GFP-α-tubulin and RFP-DCX for live green and red fluorescence microscopy. Cross-talk between different channels was none or minimal (data not shown). C, same as B except that GFP-ATAT1 was expressed instead of GFP-α-tubulin. Similar results were obtained with mCherry-ATAT1 and GFP-DCX, although mCherry-ATAT1 was more enriched in the cytoplasm than GFP-ATAT1 (data not shown). Scale bar, 40 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: Mice Lacking ?-Tubulin Acetyltransferase 1 Are Viable but Display ?-Tubulin Acetylation Deficiency and Dentate Gyrus Distortion *

    doi: 10.1074/jbc.M113.464792

    Figure Lengend Snippet: Human ATAT1 colocalizes with doublecortin-bundled microtubules. A, HEK293 cells were transfected with an expression plasmid for GFP-α-tubulin, RFP-DCX, or GFP-ATAT1 for live green or red fluorescence microscopy. B, HEK293 cells were cotransfected with the expression plasmids for GFP-α-tubulin and RFP-DCX for live green and red fluorescence microscopy. Cross-talk between different channels was none or minimal (data not shown). C, same as B except that GFP-ATAT1 was expressed instead of GFP-α-tubulin. Similar results were obtained with mCherry-ATAT1 and GFP-DCX, although mCherry-ATAT1 was more enriched in the cytoplasm than GFP-ATAT1 (data not shown). Scale bar, 40 μm.

    Article Snippet: The eGFP-ATAT1 construct was prepared from a human cDNA clone from Open Biosystems, whereas the ones for eGFP-α-tubulin and RFP-DCX were obtained from Addgene (plasmids 30387 and 32851, respectively).

    Techniques: Transfection, Expressing, Plasmid Preparation, Fluorescence, Microscopy